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Yes, the Desire streetcar in New Orleans, Louisiana ran through Bourbon Street:
The Desire line ran from Canal Street, down Bourbon Street, and to Desire Street in the 9th Ward. It then looped back to Canal Street by traveling up Royal Street. The Desire line was a popular way for people living in the French Quarter to get around.
The Desire line was made famous by Tennessee Williams' 1947 play A Streetcar Named Desire. Williams lived at 632 St. Peter St. and could hear the streetcar rumble down the tracks.
In 1948, buses replaced the streetcars on the Desire line. A restored Desire streetcar was donated to the Louisiana Tourist Development Commission in 1967. It was on display at the French Market and the Old U.S. Mint before being moved to other locations.