I have a non-immigrant B visa and have arrived in country to gain a work permit for a new job here but my education certificate has been lost. As it shall be a senior role within a large thai based company and i have held executive roles and board positions with major similar industry companies - shall i be able to get my work permit based upon previous experience and my resume?

That depends on the local labour office, some do not require others will insist, and also on the company and its reputation. Big companies tend to have lesser problems.

Might be worth to get a certified copy of your education certificate for future use.