I have 3 ponds, each is 75' x 25' x 6' so it's a bunch of gallons in total, over 200,000.
I have catfish, tilapia and koi. Luckily I'm pushing the water horizontal the 100 feet other than the pull from the pond is 3 feet deep. Yeah I've experimented with the run times but to keep the cycle flowing thru the 3 ponds (there interconnected) it's tough due to the number of fish. 24/7 has been optimum run time to maintain clear water as these are for commercial sale.